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KMID : 0355620120380010014
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Journal of Korean Association of Oral and Maxillofacial Surgeons
2012 Volume.38 No. 1 p.14 ~ p.19
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Expression of ssrA in non-pathogen-induced adaptation in the oral cavity through signal exchange with oral pathogens
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Kim Sung-Ryoul
Kwak Jae-Woo
Lee Sung-Ka
Jung Seung-Gon
Han Man-Seung
Kim Bang-Sin
Kook Min-Suk
Oh Hee-Kyun
Park Hong-Ju
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Abstract
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Introduction: This study was conducted to evaluate ssrA expression resulting from adaptation of Escherichia coli (E. coli ) to oral pathogens through signal exchange. Materials and Methods: Human cell lines Hep2 and HT29, wild-type E. coli (WT K-12), ssrA knock-out E. coli (Δ K-12), and Scleropages aureus (S. aureus ) were used. A single culture consisting of Hep2, HT29, WT K-12, and Δ K-12, and mixed cultures consisting of Hep2 and WT K-12, Hep2 and Δ K-12, WT K-12 and S. aureus , Δ K-12 and S. aureus , and Hep2, WT K-12, and S. aureus were prepared. For HT29, a mixed culture was prepared with WT K-12 and with WT K-12 and S. aureus . Total RNA was extracted from each culture with the resulting expression of ssrA, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and p53 was evaluated by Reverse transcription polymerase chain reaction (RT-PCR). Results: The expression of ssrA in a single culture of WT K-12 was lower than that observed in the mixed culture of WT K-12 with S. aureus . Greater ssrA expression was observed in the mixed culture of WT K-12 with Hep2 than in the single culture of WT K-12. The expression of NF-κB was higher in the mixed culture of Hep2 with Δ K-12 than that in the mixed culture of Hep2 with WT K-12, and was lowest in the single culture of Hep2. The expression of ssrA was higher in the mixed culture of WT K-12 with Hep2 and S. aureus than in the mixed culture of WT K-12 with Hep2. Conclusion: These results suggest that ssrA plays an important role in the mechanism of E. coli adaptation to a new environment.
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KEYWORD
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ssrA, Escherichia coli, Adaptation
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